This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.įunding: This work was supported by grants (# PJ00956901, PJ00956302 and PJ009080) from the Next-Generation Bio Green 21 Program, Rural Development Administration, Republic of Korea. Received: JAccepted: DecemPublished: January 15, 2014Ĭopyright: © 2014 Jin et al. PLoS ONE 9(1):Įditor: Reiner Albert Veitia, Institut Jacques Monod, France (2014) Production of Pigs Expressing a Transgene under the Control of a Tetracycline-Inducible System. These data show that the expression of transgenes in cloned TG pigs can be regulated by the Tet-on/off systems.Ĭitation: Jin Y-X, Jeon Y, Lee S-H, Kwon M-S, Kim T, Cui X-S, et al. The re-cloned SCNT embryos expressed the eGFP transgene under the control of doxycycline.
Two TG fibroblast cell lines were established from these TG piglets, and these cells were used as donor cells for re-cloning. PCR analysis showed that the transgene was inserted into the genome of each of these piglets. The remaining 4,101 embryos were transferred to thirty three surrogate pigs from which thirty eight cloned TG piglets were obtained. All of these embryos expressed eGFP and 15 embryos developed to blastocyst stage. Of 4,951 porcine SCNT-TG embryos generated, 850 were cultured in the presence of 1 µg/ml doxycycline in vitro. The Tet-on system was then tested in the generated porcine SCNT-TG embryos. Somatic cell nuclear transfer (SCNT) was then performed using these donor cells.
At 1 day after treatment with 1 µg/ml doxycycline, the fluorescence intensity of these cells was increased. Porcine fetal fibroblasts were infected with a universal doxycycline-inducible vector containing the target gene enhanced green fluorescent protein (eGFP). The Tet-on system was first tested in infected donor cells. The aim of this study was to produce pigs expressing a tetracycline (Tet)-inducible transgene. Transgenic (TG) pigs are widely used as models of human diseases.
Pigs are anatomically and physiologically closer to humans than other laboratory animals.
0 kommentar(er)
